| PCⅠ

Procollagen I (PCI) is a trimer composed of two identical alpha1 (I) polypeptide chains and one α2 (I) polypeptide chain. During the extracellular transformation of procollagen into collagen, procollagen propeptides, i.e., procollagen type I carboxyl terminal propeptide (PICP) and procollagen type I amino terminal propeptide (PINP), are released into the circulation. It is a precursor synthesized by osteoblasts and secreted into the extracellular space, where it undergoes a series of post-translational modifications, including propeptides cleaved by specific proteases. The propeptides are released into the circulation, and the newly formed collagen molecules assemble into a fibrous shape.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.29	1.64	1.08	0.7	0.44	0.28	0.19	0.08
Corrected OD	2.21	1.56	1	0.62	0.36	0.2	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human PCⅠ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-99	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	106

 

| Linearity

Samples were spiked with high concentrations of Human PCⅠ and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	92-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	109